Ukuvula amandla e-EGTA CAS 67-42-5: inhlanganisela yemisebenzi eminingi yezinhlelo zokusebenza zesayensi nezimboni

I-Ethylene bis(oxyethylenenitrilo)tetraacetic acid, eyaziwa nangokuthi i-EGTA CAS 67-42-5, iyinhlanganisela eguquguqukayo enezinhlobonhlobo zezinhlelo zokusebenza kumalabhorethri emithi, amakhemikhali ezinto eziphilayo, kanye nawocwaningo.Izakhiwo zayo eziyingqayizivele kanye nezinhlobonhlobo zezinzuzo ziyenza ibe isengezo esibalulekile kunoma iyiphi indawo yesayensi neyezimboni.

I-EGTA i-ejenti yokuchela evame ukusetshenziswa kuzilungiselelo zaselabhorethri ukuze i-chelate futhi ibophe ama-ion ensimbi, ikakhulukazi ama-calcium ions.Ikhono layo lokucwenga kahle ama-ion ensimbi lenza ithuluzi elibalulekile ocwaningweni oluhlukahlukene lwe-biochemical kanye nemithi.Ukwengeza, i-EGTA isetshenziswa kaningi ezinhlelweni ze-cell culture ukuvimbela ukuhlangana kwe-calcium ne-magnesium ion, okuyenza ibe yingxenye ebalulekile ocwaningweni lwe-cell biology.

Ngaphezu kwalokho, i-EGTA isetshenziswa kabanzi emkhakheni we-molecular biology kanye ne-biochemistry.Ikhono layo lokuchela ama-ion ensimbi lizinzisa ama-enzyme futhi livimbele ukusabela kwe-oxidative ye-metal-catalyzed, okwenza kube yingxenye ebalulekile yokulondolozwa nokucwaninga kwamaprotheni nama-nucleic acid.Ukusebenziseka kwayo ngezindlela eziningi ocwaningweni lwebhayoloji yamangqamuzana kuyenze yaba inhlanganisela ebalulekile kumalabhorethri emhlabeni jikelele.

Ngokungeziwe ekusebenziseni kwayo ezimbonini zemithi kanye ne-biochemical, i-EGTA idlala indima ebalulekile ezinqubweni ezihlukahlukene zezimboni.Izakhiwo zayo ze-chelating ziyenza ibe isithako esisebenzayo ekwakhiweni kwemikhiqizo yokunakekelwa komuntu siqu, okokuhlanza kanye nezixazululo zokwelashwa kwamanzi.I-EGTA inekhono lokuchela ama-ion ensimbi, isuse ukungcola futhi ivimbele ukusabela kwamakhemikhali okungafuneki, iyenze ibe yimpahla ebalulekile ezilungiselelweni zezimboni.

Sekukonke, i-EGTA CAS 67-42-5 iyinhlanganisela eguquguqukayo enezinhlobonhlobo zezinhlelo zokusebenza kuzilungiselelo zesayensi nezimboni.Izakhiwo zayo ezihlukile ze-chelating ziyenza ibe ithuluzi elibalulekile kumalabhorethri emithi, amakhemikhali ezinto eziphilayo kanye nokucwaninga, kuyilapho idlala indima ebalulekile ezinqubweni ezihlukahlukene zezimboni.Ngobubanzi bayo bezinzuzo kanye nezakhiwo eziguquguqukayo, i-EGTA iyisengezo esibalulekile kunoma iyiphi indawo yesayensi neyezimboni.Ukuthi ukuzinzisa ama-enzyme kuzilungiselelo zaselabhorethri noma ukuvimbela ukuhlangana kwama-ion ensimbi ezinqubweni zezimboni, i-EGTA iyinhlanganisela evula amandla okuqamba nokuthuthuka emikhakheni eyahlukene.